Why did we use a primary antibody that recognizes the xpress epitope tag and not the his6 tag to detect rgfp?

Answers

Answer 1

we use primary antibodies that recognize the Xpress epitope tag and not the his6 tag to detect rGFP because using antibodies that can detect his-tag will get bind to it.

Your immune system creates defensive proteins called antibodies. They bind to antigens (foreign substances), which include poisons, viruses, bacteria, and fungus, and eliminate them from your body. Your immune system creates defensive proteins called antibodies. They bind to antigens (foreign substances), which include poisons, viruses, bacteria, and fungus, and eliminate them from your body.

A protein called an antibody is created by your immune system to attack and neutralize antigens. The B cell produces antibodies (specialized white blood cells). A B cell multiplies and clones when it comes into touch with an antigen. These cloned B cells, also known as plasma cells, unleash countless quantities of antibodies into your lymphatic and blood systems.

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Related Questions

What type of anemia is caused by defective red blood cells with a shortened life span?

Answers

The name of the anemia is Hemolytic anemia which is caused by defective red blood cells.

It is simply a disorder in which red blood cells are more quickly destroyed as compared to what can be generated. The number of red blood cells decreases is called hemolysis.

It is also caused due to some infections in the body. As we know red blood cells carry oxygen and if the body has fewer number cells then proper oxygen is not carried out to the tissues and organs of the body.

If your body does not have proper oxygen, the body can't function properly. Oxygen is very important for us to function properly. Without oxygen, human life is impossible.

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A complex consisting of a single mrna molecule with several ribosomes is called a _________.
a. polysome
b. multisome
c. maxisome
d. proteosome

Answers

I believe the answer is (a) a polysome

An organism’s complexity ____ with the number of its genes; it ____ with the amount of junk dna.

Answers

An organism's complexity is directly related to the amount of junk DNA.

Most eukaryotic genomes are larger and more complex than those of prokaryotes. However, the genome size of many eukaryotes does not appear to be related to genetic complexity.

For example, the genomes of salamanders and lilies contain more than ten times the amount of DNA that is in the human genome, yet these organisms are clearly not ten times more complex than humans.

Further studies showed that in the human genome only 2.5%of the genes are coding for protein while the rest of the 97.5% of genes are non-coding genes or junk DNA.

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When the tooth pulp has symptoms of lingering pain, a diagnosis of _______ can be made.

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When the tooth pulp has symptoms of lingering pain,a diagnosis of irreversible pulpits can be made. pulpit is diagnosis by dentist, they take one or more extras to diagnosis of tooth decay and inflammation.

If inflammation builds in tooth the pulp can die. this is happens with irreversible pulpits and if once occur the pain will stop and tooth wouldn't be sensitive for cold or hot water. An infected pulp of the tooth will not heal on its own, so a root canal is suggested by dentist.

When tooth pulpit damage the tooth then removal of the tooth, relieving the pain of irreversible pulpits is by draining into tooth removing the pulpit and cleaning the root canal.

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If there is an excess of reactive oxygen species (ross) in a system, where would the problem least likely be found?

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This condition is known as a super oxide in which mutated enzyme extremely functional because it helps in production of cells .

when unstable molecules that contains oxygen and that easily react with other molecules in the cells. a build up of reactive oxygen species and cells causes damage of DNA ,RNA & protein ,which cause cell death.

super oxide refers various toxic oxygen containing free radicals such as monovalant anion O2 (negative) or that compound containing potassium super oxide KO2. reactive oxygen can cause damage to the basic building block of the cell. DNA damage can occur in the form of double stranded breaks as a result of reactive oxygen induced conversion of guanine to 8-oxo guanine.

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The structures of the limbic system are particularly important in group of answer choices emotion and learning. sensation. motor control. regulation of hunger and thirst.

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The structures of the limbic system are particularly important in  emotion and learning and is denoted as option A.

What is Limbic system?

This is referred to the part of the brain located in both sides of the thalamus and are involved in behavioral and emotional responses such as feeding, reproduction etc.

This system also controls the emotion and learning aspect needed for our survival thereby making it the most appropriate choice.

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From deep (innermost) to superficial (outermost), the layers of the organs of the alimentary canal are? 1) mucosa; 2) muscularis externa; 3) serosa (adventitia); 4) submucosa?

Answers

The correct order of the layers of the organs of the alimentary canal is mucosa, submucosa, muscularis externa, and serosa. The correct option is B.

What is alimentary canal?

The alimentary canal is a long tube of organs that allows food to pass through the body.

It encompasses the esophagus, stomach, and intestines and runs from the mouth to the rectum. The digestive tract of an adult is approximately 30 feet long.

Smooth muscle fibers or cells are long and spindly in shape. Peristalsis is the prime objective of these muscle fibers.

Mucosa, submucosa, muscularis externa, and serosa are the correct layers of the alimentary canal organs.

Thus, the correct option is B.

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Your question seems incomplete, the probable options are:

A) 3, 2, 4, 1B) 1, 4, 2, 3C) 1, 4, 3, 2D) 1, 2, 4, 3

When muscle cells undergo anaerobic respiration, they become fatigued and painful. this is now known to be caused by:_____.

Answers

The correct option is C.

The skeletal muscle will be painful and fatigued and the caused it  build up of lactate.

What is skeletal muscle?

Of the three major muscle tissues in the human body, skeletal muscle is one of them. The connective tissue sheaths that cover each of the thousands of muscle fibers that make up each skeletal muscle. The term "fasciculi" refers to the distinct bundles of muscular fibers that make up skeletal muscles.

What is  lactate?

It is a test that assesses the level of lactic acid, often known as "lactate," in your blood. Muscle cells and red blood cells produce this acid. When food is converted into energy by your body, it forms. In times of low oxygen levels, your body uses this energy.

Lactic acid has long been believed to be the cause of this acid buildup, known as acidosis, which is linked to the burning sensation experienced after vigorous exercise.

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I understand that the question you are looking for is:

When skeletal muscle cells undergo anaerobic respiration, they become fatigued and painful. This is now known to be caused by _____

A) a buildup of pyruvate.

B) an increase in ethanol.

C) a buildup of lactate.

D) an increase in sodium ions.

E) an increase in potassium ions.

It is now understood that the painful and fatigued symptoms of anaerobic respiration in muscles and skeletal muscle cells are brought on by a buildup of lactate. The accumulation of lactate will cause the skeletal muscle to become sore and exhausted.

What is Anaerobic respiration?

Glucose is metabolized anaerobically, or without oxygen. Energy from glucose is transferred to the cell through the chemical process. Lactic acid is the end product of anaerobic respiration rather than carbon dioxide and water.

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Which is a function of the golgi apparatus?
a. cell respiration
b. protein modification
c. drug detoxification
d. digestion of organic matter inside the cell

Answers

Golgi apparatus also called as golgi complex or golgi body ,cell membrane bound organelle of eukaryotic cells. Its a factory in which proteins modification received from the ER.

It helps in process and lipid molecules protein destined to be extorted from the cells. Camillo golgi body appears as a dries of stacked membranes. The gogli is located right near the nucleus.

The protein modification is cellular organelle responsible for the trafficking of proteins to other organelles. Proteins translated within the rough endoplasmic reticulum are transferred to golgi. The golgi enzymes catalyze the addition or removal of sugars for cargo protein.  

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Food elements are broken down into forms that can be transported to and absorbed by cells. This process is called?

Answers

Answer:

Digestion

Explanation:

The breakdown of foods into forms that can be transported to cells and absorb into these cells(this process has two parts): Mechanical(breaks down large pieces of food into smaller ones) and Deglutition(swallowing).

You will suffer a variety of painful symptoms, including indigestion, bloating, and stomach discomfort, if your digestive system isn't in good shape.

What are the important component of digestion?

The process of physically and enzymatically converting food into chemicals that may be absorbed into the bloodstream is called digestion.

The gastrointestinal tract is composed of the mouth, esophagus, stomach, pancreas, liver, gallbladder, small intestine, large intestine, and anus.

The secret to comprehending how digestion functions is to be aware of how these organs cooperate to break down food. Beginning in the mouth, the digestive process.

Therefore, The digestive system is crucial to your overall health since it provides your body with nutrients.

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The _____ secretes a hormone that increases the body's metabolic rate, breathing rate, and heart rate.

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The thyroid gland secretes a hormone that increases the body's metabolic rate, breathing rate, and heart rate.

The thyroid gland makes two hormones that it secretes into the bloodstream. One is called thyroxine ,this hormone contains four atoms of iodine and is often called T4. The other is called triiodothyronine, which contains three atoms of iodine and is often called T3. Activation of thyroxine (T4), to the active form, triiodothyronine (T3), by 5′-deiodinase type 2 (D2) is a key mechanism of TH regulation of metabolism. D2 is expressed in the hypothalamus, white fat, brown adipose tissue (BAT), and skeletal muscle and is required for adaptive thermogenesis.

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What are the consequences of learning on the brain and on future execution of behavior by that brain?

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Human brain is always learning whenever it processes information.Human babies are the best example of learning since birth.The aging brain responds to learning the same way a child's brain does grows new task- specific synapse that can be recruited for other uses.

These are the consequences of learning on the brain and on future execution of behavior by that brain.

What are the consequences of learning of brain?

When brain learns something new it will help to develop cognitive function like concentration, attention and chance of dementia decreases.

When the brain matures, fibers are developed in the brain becomes interconnected and these interconnected neurons are very essential in the formation of memories and new and previous learning interconnected.

Therefore,human brain is always learning  when it is processed.

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Extending from norway to the pacific coast of russia is a great expanse of ________ climate, often referred to as the taiga climate.

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Extending from norway to the pacific coast of russia is a great expanse of subarctic climate, often referred to as the taiga climate.

What is Taiga climate?

This type of climate observed in the northern hemisphere and absent in the southern hemisphere.

It is otherwise named as boreal Climate or Siberian Climate or Cool Temperate Continental Climate or Continental Sub-Polar Climate.

The temperature of this climatic regions include range from 20-25 °C  during summer whereas in winters are cold that is 30-40 °C below freezing.

The range of annual precipitation 38 cm to 63 cm.

Hence, from norway to the pacific coast of russia is a great expanse of subarctic climate.

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Amino acids are used to make _____, which are responsible for escorting substances into and around the body as well as across cell membranes

Answers

Answer:

protein

Explanation:

Amino acids are joined together to form proteins. The main function of amino acids is to serve as building blocks for proteins

When a photon is absorbed by chlorophyll, the energy level of some of the electrons in the pigment is raised. which of these are possible after that?

Answers

An electron is stimulated from the ground state to a higher energy level as a result of the absorption of a photon in the PAR range by a photosynthetic pigment.

These excited states can go from one pigment molecule to another, but because moving from carotenoids and chlorophyll b to chl an is energetically less favourable, excited states created on those pigment molecules end up on chl a.

What is a Photosystem ?

The functional components of photosynthesis are called photosystems, and they are identified by specific pigment organisation and association patterns.

Photosystems are responsible for absorbing and transferring light energy, which implies transferring electrons. The thylakoid membranes contain photosystems in terms of physical location.

Photosystem I and Photosystem II have been identified in plants and algae. Each of them consists of chlorophyll molecules, accessory pigments, proteins and other molecules.

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Aerobic respiration creates lactic acid as a by prodcut but prodcues far more atp than respiration.
a. true
b. false

Answers

False, aerobic respiration creates lactic acid as a by product but produces far more ATP than respiration. When lactic acid is ferment by certain bacteria which includes the bacteria in yogurt and also by human muscles cells when they are worked hard and fast.

In aerobic cell respiration which includes glycolysis + the kerbers cycle = respiratory electron transport that produce 36 ATP /glucose consumed. Aerobic respiration cell are 18 times more efficient than anaerobic cell respiration because in aerobic respiration more ATP than anaerobic respiration due to the complete oxidation of glucose to CO2 and water .

Now O2 acts as terminal electron accept in this electron transport chain and gets reduced to water.ATP are produced by oxidation phosphorylation in the electron transport chain.

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What is the correct sequence of structures encountered by neural information as it travels along a neuron?

Answers

The correct sequence of structures encountered by neural information as it travels along a neuron is Cell body > Axon > Nerve terminal.

How does neural information travels along a neuron?

The neural information also known as the impulse is generated and transmitted through the nerve cells. The impulse is received by dendrites, which then send it to the cell body.. From the cell body, the impulse flows through the axon and then reaches the nerve terminal. The subsequent neuron receives the impulse through the synaptic connection.

The correct sequence of impulse travel through neurons is cell body to axon to the nerve terminal.

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Activity of the digestive system is generally increased by _____________ nervous stimulation.

Answers

Answer:

nervous stimulation. parasympathetic.

Explanation:

The pluripotent, undifferentiated cells in the bone marrow that give rise to the formed elements are called _____ cells

Answers

The pluripotent, undifferentiated cells in the bone marrow that give rise to the formed elements are called stem cells.

Pluripotent stem cells have the ability to undergo self-renewal and to give rise to all cells of the tissues of the body. However, this definition has been recently complicated by the existence of distinct cellular states that display these features.

There are two types of pluripotent stem cells :

Embryonic stem cells (ESCs)Induced pluripotent stem cells

Embryonic stem cells are derived from the inner cell mass (ICM) of pre-implantation embryos and can be indefinitely maintained and expanded in the pluripotent state in vitro. Pluripotent stem cells can also be obtained by inducing dedifferentiation of adult somatic cells through a recently developed in vitro technology, known as cell reprogramming.

Similarly to  Embryonic stem cells , Induced pluripotent stem cells can be expanded indefinitely and they are capable to differentiate in all the derivatives of the three germ layers.

Another intriguing features of being pluripotent is its reliance on gene expression heterogeneity.

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In a bacteria, a subunit of RNA polymerase called ____ recognizes-10 sequence in the promoter and binds RNA polymerase there.

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In a bacteria, a subunit of RNA polymerase called α subunit recognizes-10 sequence in the promoter and binds RNA polymerase there.

What is RNA polymerase?

RNA polymerase is defined as the type of enzyme found in Ribonucleic acid bacterias which helps in copying a DNA sequence into an RNA sequence.

The RNA polymerase are also known as the main enzyme for transcription in bacteria.

The subunits of RNA polymerase enzyme include the alpha subunit.

The alpha subunit is known to be able to recognise 10 sequence in the promoter and binds RNA polymerase there.

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Hich physiologic change increases cardiac work but does not enhance cardiac output?

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Increased afterload physiologic change increases cardiac work but does not enhance cardiac output.

What about cardiovascular system?Heart and blood vessels, which make up your cardiovascular system, deliver oxygen and nutrition to your body's organs so they can function. Blood vessels also transport waste such as carbon dioxide to be disposed.Conditions affecting the heart or blood vessels are collectively referred to as cardiovascular disease. It is frequently associated with atherosclerosis, an accumulation of fatty deposits in the arteries that increases the risk of blood clots.The heart, blood arteries, and blood make up the cardiovascular system. Its main job is to carry deoxygenated blood back to the lungs and to carry nutrients and oxygen-rich blood to all regions of the body.The most typical cause of coronary artery disease is atherosclerosis, which is a buildup of fatty plaques in your arteries. Atherosclerosis can be brought on by unhealthy lifestyle choices such smoking, being overweight, not exercising, and eating poorly.

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please i need help!!!

Answers

Answer:

the answer is d

Explanation:

photosynthesis is a process in plants to produce o2 and energy rich compounds

Does the overall shape of lucy’s pelvis resemble the human pelvis or the chimpanzee pelvis more? do not think of the size, but compare the outlines of each bone

Answers

In human pelvis the wings of the ilium extended from either side of the pelvis , in chimpanzee pelvis the wings are extend from the back because chimpanzee is tall.

Lucy and other species could walk well because their hip and knee joints were similar to humans." like human's,like chimp's".Her small skull with long arms and conical rib cage are like an ape's but she has a more human for example- spine, pelvis and knee due to walking upright.

According to Johnson - Lucy was either a small member of genus homo or small Australopithecus. When comparing Lucy , its shows an intermediate curvature between those of modern human and chimpanzees.

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Many bacteria facilitate the uptake of iron by secreting low molecular weight molecules, called ___________, to form complexes with the iron that can then be readily transported into the cell.

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Many bacteria facilitate the uptake of iron by secreting low molecular weight molecules, called siderophores, to form complexes with the iron that can then be readily transported into the cell.

What are siderophores?Siderophores are natural or synthetic, low molecular weight molecules which have a high binding affinity to iron.Microorganisms like bacteria and fungi secrete these molecules to enhance iron uptake through the cell.Iron is abundant in the earth’s crust but it is not readily available in soluble form.Siderophores can chelate iron (form water soluble complexes). This complex is then taken up by the cell through membrane receptors.These are the strongest and largest class of iron chelators known thus providing the much needed iron to the microorganisms.Siderophores can also form complexes with aluminium, copper, zinc, manganese, cadmium, lead, etc.

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Students are measuring the rate of enzyme activity. The graph below shows the rate of enzyme action as they increase the temperature

Answers

The subtrate will no longer fit within the enzyme because the heat will cause the enzyme to denature (deform).

Enzyme

Proteins called enzymes serve as biological catalysts (biocatalysts). Chemical reactions are sped up by catalysts. Substrates are the molecules that enzymes can interact with, and the enzyme changes the substrates into new molecules known as products. Almost all cellular metabolic activities require enzyme catalysis in order to proceed at speeds quick enough to maintain life. Enzymes are necessary to catalyze specific steps in metabolic pathways. Enzymology, the study of enzymes, and the pseudoenzyme analysis area acknowledge that some enzymes have lost the capacity to perform biological catalysis during evolution, which is frequently reflected in their amino acid sequences and peculiar "pseudocatalytic" capabilities.

Students are measuring the rate of enzyme activity. The graph below shows the rate of enzyme action as they increase the temperature

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Some citizens are concerned about the use of genetically modified plants because they:_____.

Answers

Some citizens are concerned about the use of genetically modified plants because they cause allergic problems.

Genetic engineering is a specific form of gene technology that modifies the genetic code of living things like plants, animals, and microorganisms. The potential for adverse consequences on the human body posed by GM foods is the biggest danger. It is thought that eating these genetically modified crops can lead to the emergence of illnesses resistant to antibiotic treatment.

The transmission of antibiotic resistance, toxicity, and allergenicity are the three main health impacts of GM foods that raise the most concern. When blown into a natural setting, pollen from a genetically modified crop that carries a pesticide may kill nontarget creatures. Genetically engineered crop pollen can contaminate non-genetically modified crop kinds.

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Lab mice are bred such that every mouse is literally a clone of any other mouse. Yet, resilience arises regularly in a fraction of the mice during CSDS studies. If they're all clones, but we always find a resilient population, what does that say about how resilience works?

Answers

You may heard of the term mutation. Even during cloning, which is done by the process of DNA replication. Certain agents referred to as mutagens bring about changes in the chromosomal constitution, or how certain genes are arranged on the chromosome. Some physical factors include X rays and gamma rays, while some chemical factors include EMS and nitrogenous gases.

Bowing to your opponent before a jiu jitsu match would be considered a ________ movement.

Answers

Answer:

voluntary, you did it by will

Explanation:

The sensory receptors, neurons and pathways make up the ________ division of the nervous system

Answers

Answer:

afferent division

Explanation:

Receptors, sensory neurons, and sensory pathways make up the afferent division of the nervous system.

Match the term to its description. (4 points) c 1. astronomical body a 2. comet b 3. galaxy d 4. moon a. an object in space b. a system of stars held by gravitational attraction c. a celestial object consisting of a nucleus of ice and a stream of gas and dust d. an astronomical body that orbits around a planet

Answers

Astronomical body - An object in space

Galaxy - A system of stars held by gravitational attraction

Comet - A celestial object consisting of a nucleus of ice and a stream of gas and dust

Moon - An astronomical body that orbits around a planet

What are different Astronomical bodies ?

The astronomical bodies or the space bodies are the entities and the substances that are present in space. Some astronomical bodies are

Galaxy: It is the system of stars that are held together with the gravitational pull.

Comet: These are the celestial bodies in space that are consist of ice, gas, and dust.

Moon: It is the outer space body that revolves around the earth.

Astronomical body: It is an object that is present in space.

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Ramiro was on a two day road trip.on the first day he drove at an average speed of 40 mph.on the second day he drove at an average of 60 mph.if he drove 2 hours longer and went 20 miles farther on his first day find the total distance ramiro traveled on his road trip. Types of governments in south asia Circle A is shown. Secant W Y intersects tangent Z Y at point Y outside of the circle. Secant W Y intersects circle A at point X. Arc X Z is 105 degrees and arc W Z is 175 degrees.In the diagram of circle A, what is the measure of XYZ?357075140Circle A is shown. Secant W Y intersects tangent Z Y at point Y outside of the circle. Secant W Y intersects circle A at point X. Arc X Z is 105 degrees and arc W Z is 175 degrees.In the diagram of circle A, what is the measure of XYZ?357075140 What past, present or future information can be gained by studying sedimentary rocks? (choose all that apply; in this case three apply) What form of communication does the national fire fighter near-miss reportign system provide for reporting and accessing situations that could have resulted in injuries or even death? The graph of a function g is shown below.Use the graph of the function to find its average rate of change from x=-2 to x=4.Simplify your answer as much as possible.1-10 Nationally, patients who go to the emergency room wait an average of 7 hours to be admitted into the hospital. Do patients at rural hospitals have a lower waiting time? The 13 randomly selected patients who went to the emergency room at rural hospitals waited an average of 6.3 hours to be admitted into the hospital. The standard deviation for these 13 patients was 1.3 hours. What can be concluded at the the = 0.01 level of significance level of significance?For this study, we should use Select an answerThe null and alternative hypotheses would be: H0:?Select an answerH1:?Select an answerThe test statistic ? = (please show your answer to 3 decimal places.)The p-value = (Please show your answer to 4 decimal places.)The p-value is ?Based on this, we should Select an answer the null hypothesis.Thus, the final conclusion is that ...The data suggest the population mean is not significantly lower than 7 at = 0.01, so there is statistically insignificant evidence to conclude that the population mean waiting time to be admitted into the hospital from the emergency room for patients at rural hospitals is equal to 7 hours.The data suggest the populaton mean is significantly lower than 7 at = 0.01, so there is statistically significant evidence to conclude that the population mean waiting time to be admitted into the hospital from the emergency room for patients at rural hospitals is lower than 7 hours.The data suggest that the population mean awaiting time to be admitted into the hospital from the emergency room for patients at rural hospitals is not significantly lower than 7 hours at = 0.01, so there is statistically insignificant evidence to conclude that the population mean waiting time to be admitted into the hospital from the emergency room for patients at rural hospitals is lower than 7 hours. According to the text, the flsa states that a child must be at least how old to work in most nonfarm jobs that have not been identified as hazardous either in the law or by the secretary of labor? What term is best described as the rate of a chemical reaction at any instant in time? Use the equation to complete an algebraic proof that proves the answer is x = 7/6. Write your proof in your journal and upload your answer. You will be awarded 5 points for the statements and 5 points for the reasons. I need the algebraic proofs please!!! How does Kittay define doulia? a. the public responsibility to provide care for dependency workers, whose work can be emotionally and physically depleting b. depending on others in times of vulnerability c. Interpersonal, one-on-one acts of care for those who do dependency work d. a public moral sentiment of justice Cattle egret follow herds of cattle and feed on both the insects stirred by the movement of the cattle and the biting insects that irritate the cattle. this is an example of:______. A 15. 0 ampere current flows through a 4. 75 m section of wire, perpendicular to a 0. 250 t magnetic field. what is the magnitude of the force exerted by the field on the wire section? For a certain company, the cost function for producing x items is C(x)=50x+100 and the revenue function for selling x items is R(x)=0.5(x100)2+5,000 . The maximum capacity of the company is 120 items.The profit function P(x) is the revenue function R(x) (how much it takes in) minus the cost function C(x) (how much it spends). In economic models, one typically assumes that a company wants to maximize its profit, or at least make a profit!Answers to some of the questions are given below so that you can check your work.Assuming that the company sells all that it produces, what is the profit function?P(x)= Read the quotation by Adam Smith.According to Adam Smith, how does self-interestbenefit society?D It causes people to seek out high-paying jobs.It encourages people to be more benevolent.It causes people to behave more cautiously.O It encouragesemployers to pay high wages. Which health education and promotion theory is based on the underlying principle that accurate information enables better health choices? A map uses a scale of 1cm = 5 miles. In actualdistance, the entrances totwo parks are 24 milesapart. How far apart arethey on the map? . An aeroplane takes 30 min to travel from Biratnagar to Kathmandu. If the air distance betweenthe Biratnagar and Kathmandu is 450 km. Find the average velocity of the plane. Why do you think omar stayed in bed for four weeks after he was discharged from the hospital? The area of the trapezoid is 40 square units.A trapezoid is shown. The lengths of the bases are 6 and 10, and the height of the altitude is h.What is the height of the trapezoid?3 units5 units10 units12 units