In the DNA isolation process, ice cold 95% ethanol is used to break down the protein complexes and allow the DNA molecules to easily precipitate.
What is separation and isolation of DNA fragments?
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis.Since, DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix.The most commonly used matrix is agarose which is a natural polymer extracted from sea weeds.The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves.The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). We can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.To learn more about DNA isolation process: brainly.com/question/18316109
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A member of the ________ has been implicated as having a role in obesity.
a. adenoviruses.
b. parvoviruses.
c. herpesviruses.
d. poxviruses.
e. both herpesviruses and poxviruses.
According to the research, the correct option is a. A member of the adenoviruses (a) has been implicated as having a role in obesity.
What are adenoviruses?They are pathogens that are characterized by not having an external cover and that cause respiratory and intestinal tract diseases.
An adenovirus called Adenovirus 36 is identified as one of the causes of obesity, this pathogen produces an accumulation of adipose tissue due to its high adipogenic activity, which simply implies that it promotes fat deposition and growth of adipose or fatty tissue.
Therefore, we can conclude that the correct option is a. A member of the adenoviruses (a) has been implicated as having a role in obesity.
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Biomolecules are made from simple monomers, yet you find a very high level of diversity. What chemical features make diversity in each category of biomolecules possible?
Chemical features like 3 dimensional structure and chemical bonding are responsible for diversity in biomolecules although they are made from simple monomers.
In the case of carbohydrates, both starch and glycogen are made up of glucose monomers yet they differ in the functions which they perform in our body. This difference is because of the chemical bonding and the 3 dimensional structure.
Similarly in protein biomolecules, amino acids act as the monomers but they differ in function due to their 3 dimensional structure. Nucleotides are the building blocks of both RNA and DNA. RNA and DNA perform different functions in the body. DNA stores ad transfers genetic information while RNA is responsible for directly coding for amino acids.
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In 2008, which country's greenhouse gas emissions surpassed those of the united states?
China's greenhouse gas emissions surpassed those of the united states.
Due to rising energy consumption brought on by an expanding economy, China has surpassed the United States as the largest emitter of carbon dioxide, the primary greenhouse gas. The United Kingdom was the leading emitter of CO2 in 1850, with emissions about six times higher than those of the United States, which came in second.
The final three countries on the list of top emitters were France, Germany, and Belgium. China was the world's top emitter in 2011, followed by the US, India, Russia, and Japan. It's interesting to note that while being the second-largest emitter in the world in both years, the United States' emissions in 2011 were 266 times more than those in 1850.
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The complex of dna and protein that makes up a eukaryotic chromosome is properly called __________.
The complex of DNA and protein that makes up a eukaryotic chromosome is properly called chromatin .
Chromatin is a complex of DNA and proteins that forms chromosomes within the nucleus of eukaryotic cells.
What is the chromatin and its function?
To create chromosomes, chromatin fibers are coiling and condensing. Numerous cellular functions, including DNA replication, transcription, DNA repair, genetic recombination, and cell division, are made possible by chromatin.What is chromatin in cell?
Chromosomes in eukaryotic cells are made of chromatin, a compound of DNA and proteins. Nuclear DNA is extremely compressed and wrapped around nuclear proteins in order to fit inside the nucleus; it does not exist as free linear strands.Where is chromatin found?
Chromatin is a genetic material or a macromolecule comprising DNA, RNA, and associated proteins, which constitute chromosomes in the nucleus of a eukaryotic cell. This chromatin is located within the cell nucleus.Learn more about chromatin
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Characid fishes are found naturally only in south america and africa. Fossils of these fish are not found on any other continents. What is the most likely explanation of this distribution pattern?
Characid fishes arose prior to the separation of the African and South American continents us the most likely explanation of this distribution pattern.
What is Characid fishes?The family Characiformes, sometimes known as the characids or characins, includes freshwater subtropical and tropical fish. The term "characins" has been used historically, but scientists now prefer the term "characids" to represent the group's status as a largely monophyletic family rank group.The order Characiforms contains the Characidae family. The bodies of characiforms are virtually always scaled, they have well-developed teeth, a pelvic fin, and typically an adipose fin.There are many different types of environments that these fish call home. From southwest Texas and Mexico through Central and South America, they are native to the Americas. Although many of these fish are found in rivers, some, like the blind cave tetra, actually live in caves.Learn more about Fossil here:
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Question: Characid fishes are found naturally only in South America and Africa. Fossils of these fish are not found on any other continents. What is the most likely explanation of this distribution pattern?
A) Characid fishes arose prior to the separation of the African and South American continents
B) Characid fishes arose in the South Atlantic Ocean and migrated to Africa and South America
C) These fishes arose in either Africa or South America and migrated across the South Atlantic Ocean to another continent
D) Convergent evolution is responsible for the distribution of characin fishes
By which proposed molecular mechanism does ca2 promote fusion of synaptic vesicles?
Through binding and inducing changes in synaptotagmin which causes the plasma membrane to curve, thus, facilitating fusion.
Ca2+ triggers synaptic vesicle exocytosis, therefore, releasing the neurotransmitters contained in the vesicles and initiating synaptic transmission. Synaptotagmin (syt) is a Ca2+ sensor which can induce fast and synchronous neurotransmitter release.
Synaptic transmission amidst pre- and post-synaptic neurons occurs when the pre-synaptic neuron terminal is temporarily depolarized upon an action potential, thereby, opening Ca2+ channels near the active zones of synapses. Ca2+ flows into the cytoplasm as the extracellular Ca2+ concentration is much higher than the cytoplasmic concentration . Successively, Ca2+ triggers fusion of neurotransmitter-filled synaptic vesicles with the presynaptic membrane in less than a millisecond.
Neurotransmitter molecules are released into the synaptic cleft upon fusion, which then bind to the receptors located in the postsynaptic membrane.
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Once habituation occurs, a new stimulus causes responsiveness to return to a high level, an increase called?
Once habituation occurs, a new stimulus causes responsiveness to return to a high level. This increase is called recovery.
Habituation is one of the simplest and commonly chosen forms of learning. It lets people tune out unnecessary stimuli and focus on the things that matter. Habituation is something that is happening on a daily basis at the time of any kind of learning but the person is usually unaware of it.
For example, if you are studying mathematics with music on it may feel distracting in the beginning, but habituation lets you tune out the noise and focus on what you are trying to learn.
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Consider an animal cell that has eight chromosomes (four pairs of homologous chromosomes) in g1 phase. this cell has ________ sister chromatids and _________ centrioles
Consider an animal cell that has eight chromosomes (four pairs of homologous chromosomes) in g1 phase. this cell has 16 sister chromatids and 4 centrioles.
What is chromosomes?A chromosome is a lengthy DNA molecule that contains all or a portion of an organism's genetic code. Histones, which serve as packing proteins for the majority of eukaryotic chromosomes, work with chaperone proteins to attach to and condense the DNA molecule in order to preserve the integrity of the molecule.A component that can be found in a cell's nucleus. There are typically 23 pairs of chromosomes in each cell.Long segments of DNA are carried by chromosomes, which are organelles that can be found in the nucleus of cells. It serves as the foundation for the human body. Additionally, proteins found in chromosomes aid in the appropriate structure of DNA.Human anatomy is created and maintained by your DNA. Genes are sections of your DNA that give you the physical traits that set you apart from others.
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How are the reproductive cycles of a fungus and a pteridophyte similar?
Fungi of the phylum Basidiomycota form mycorrhizal associations with orchids, a type of flowering plant. How do these associations benefit both the fungi and the orchids?
Fungus and a pteridophyte organisms produce haploid spores and exhibit diploid and haploid stages.
What is haploid stages?After mitosis, diploid cells are created that have the same genetic makeup as their parent cell. After meiosis, due to genetic cross-over, the haploid cells are not genetically identical to their parents. The sporophytic stage of the lifecycle is the diploid stage. The gametophytic stage of the lifecycle is the haploid stage.A diploid zygote is created when gametes produced by the multicellular haploid stage (the gametophyte) join together during mitosis. The zygote grows into a multicellular, mature diploid organism called a sporophyte, which uses meiosis to produce haploid spores.Any member of the eukaryotic group of organisms, which also includes the more well-known mushrooms and microbes like yeast and mold, is referred to as a fungus.Learn more about haploid stage here:
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What should you do if you need to leave the laboratory temporarily in the middle of your work?
While leaving the laboratory temporarily in the middle of your work remove your lab coat and gloves.
Laboratory safety:Washing your hands is the final thing you should do before leaving the lab after an experiment. Since most chemicals are somewhat harmful, wash your hands before you leave. After taking the necessary measures, inform the teacher.
With its risky processes, hazardous chemicals, and fire threats, the science laboratory is inherently unsafe. Avoid coming into contact with chemicals directly. Never taste, smell, or inhale lab chemicals. After taking off your gloves and before leaving the work area, wash your hands and arms thoroughly with soap and water. In a laboratory, never consume food or liquids, chew gum or tobacco, light up, or use cosmetics. These fundamental safety offer guidance on conduct, cleanliness, and safety to prevent laboratory mishaps.
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HELP HELP PLEASEEE!!!! ASAPPP Sherwin has been experiencing panic attacks when he leaves his home. Which biomedical treatment may be used to treat his condition?
Anti-psychotic medications
Anti-anxiety medications
Mood stabilizers
Anti-depressant medications
The correct answer is Anti-psychotic medications.
Anti-psychotic medicationsDrugs known as antipsychotics can lessen or eliminate psychotic symptoms like delusions and hallucinations (seeing or hearing something that is not there). The primary class of pharmaceuticals used to treat schizophrenia is known as antipsychotic medications, which were once classified as major tranquilizers and neuroleptics. The psychosis that can be brought on by bipolar disorder, depression, or Alzheimer's disease is also treated with them. Antipsychotics are also used to treat Tourette syndrome, bipolar disorder, and anxiety disorders by stabilizing moods, lowering anxiety, and reducing anxiety.
Antipsychotic medications can assist a person with acute psychosis calm down and make sense of their bewilderment within hours or days, but it may take them up to four or six weeks for them to fully take effect.
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What conditions are necessary in order to use a t-test to test the differences between two population means?.
The two samples must be independent and each must be drawn at random from any population. The two samples must be independent and each must be drawn at random from a healthy population.
At the very least, nominal scale data are required. There must be normal populations. There must be knowledge of the two population standard deviations. The samples depend on one another.
What is nominal scale?
A nominal scale is a measurement scale that is used to categorize events or things into distinct groups. This type of scale only needs one-of-a-kind identifiers to mark each separate category instead of requiring the usage of numerical values or categories ranked by class.
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Which class of dot and iata hazardous materials encompasses human and animal pathogens?
From the DOT and IATA classifications, hazardous materials encompassing human and animal pathogens are marked as category B.
What is DOT and IATA?The term DOT stands for Department of Transportation while the term IATA stands for International Air Transport Association (IATA). These two agencies are quire responsible for the shipping of hazardous substances.
These agencies tend to classify hazardous substances into different groups and this informs how they are to be transported from one place to another.
Hence, according to the DOT and IATA classifications, hazardous materials encompasses human and animal pathogens are marked as category B.
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The examination of blood film should be performed at _____ to detect microfilariae.
Answer:
Night
Explanation:
Usually, a microfilariae blood test is conducted at night to coincide with the appearance of microfilariae.
In response to a specific stimulus, autonomic reflex arcs can trigger which responses?
In response to a specific stimulus, autonomic reflex arcs can trigger smooth muscle contraction, cardiac muscle contraction and secretion by glands.
An element of the peripheral nervous system that controls unconscious physiological functions is the autonomic nervous system. The reflex arc is a unique kind of neural circuit that starts with a sense neuron at a receptor (for instance, a pain receptor in the fingertip) and ends with a motor neuron at an effector (e.g., a skeletal muscle).
Heart rate, digestion, respiration, salivation, sweat, pupillary dilation, micturition (urination), and sexual desire are all influenced by the ANS. Because sympathetic and parasympathetic activation are recruited quickly and synchronously, autonomic reflex arcs are complicated in character.
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An unknown bacterium produces colorless colonies when inoculated onto an emb plate. predict what you would see if you inoculated that same unknown onto the mannitol salt agar media?
The colourless colonies are only the gram-negative bacterium that will grow as the selective media Eosin Methylene Blue (EMB) followed by mannitol salt agar (MSA), which will only allow one type of microorganism to grow.
Eosin Methylene Blue (EMB) agar is a differential microbiological medium that gives a colour indicator to distinguish between organisms that digest lactose and those that do not while significantly inhibiting the development of Gram-positive bacteria. In laboratories, the selective medium is crucial for differentiating gram-negative microorganisms.
Both EMB (Eosin methylene blue) and Mannitol Salt Agar (MSA) are selective in that they only permit the growth of Gram-negative bacteria.
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What substance decreases during a reaction and can be used to measure the activity of an enzyme?
Answer:
substrate
Explanation:
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After the mordant has been added, gram-positive organisms are stained __________ and gram-negative organisms are stained __________.
In gram staining After the mordant has been added, gram-positive organisms are stained purple-violet and gram-negative organisms are stained violet black.
Gram-positive cells will be stained a purplish-blue tint at the conclusion of the gram staining process. Crystal violet is also taken up by gram negative cells, and much like in gram positive cells, the iodine forms a crystal violet-iodine compound in the cells.Gram's Iodine serves as the mordant. This forms a substantial complex that binds to the crystal violet and attaches to the cell membrane.In Gram negative bacteria, there is a thin peptidoglycan layer sandwiched between two thin cell membranes. The bacteria are first rinsed with crystal violet, a purple stain, then iodine to perform a Gram stain. Iodine and crystal violet combine to generate massive complexes that adhere to cells and color them purple.learn more about gram staining here: https://brainly.com/question/13561218
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You dropped your 24-hour-old mannitol salt agar and macconkey agar plates of staphylococcus aureus. How would you visually be able to tell the difference now?
S.aureus on mannitol salt agar will have a yellow halo (fermentation), whereas the crystal violet on the MacConkey agar would inhibit its growth.
Staphylococcus aureus:About 30% of people have a type of bacteria called Staphylococcus aureus in their noses. Staphylococcus often doesn't hurt anyone, although occasionally it might result in infections. These staphylococcus infections, which can be serious or lethal in healthcare facilities, include:
When bacteria enter the bloodstream, it is called bacteremia or sepsis.Pneumonia is a disease that primarily affects persons with underlying lung conditions, especially those who are using mechanical ventilators.Endocarditis is a heart valve infection that can cause heart failure or stroke.Osteomyelitis is a bone infection that can be brought on by staphylococcus bacteria entering the circulation or by direct contact, such as after trauma (such as a foot puncture wound or IV drug usage).Learn more about staphylococcus here:
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Question 7: what conclusions can you make about how a change in the condition of the ocean affects the different species in coral reefs?
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What are characteristics associated with the two types of nephrons? check all that apply
Cortical nephrons occupy 85% of all nephrons where as Juxtamedullary nephrons make up about 15% of all nephrons. Thus, option B and C are correct.
What is Nephron?It is the functional unit of kidneys. Human kidneys contains around 1 to 1.5 million nephrons.
Nephrons are have two main regions, one is glomerular called as Bowman's capsule and second one is the tubular portion.
The main function are removal of waste material like solid wastes, excess water from the blood. It convert blood into the urine, reabsorb, secrete, and excrete of number of nonessential substances.
Hence option B and C are correct.
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Your question was incomplete. The probable question was
What are characteristics associated with the two types of nephrons? check all that apply
A. Cortical nephrons make up about 15% of all nephrons.
B. Cortical nephrons make up about 85% of all nephrons.
C. Juxtamedullary nephrons make up about 15% of all nephrons.
D. Juxtamedullary nephrons make up about 85% of all nephrons.
Which receptor fires continuously as long as a stimulation is applied?
Type I cutaneous mechanoreceptor (Merkel disc), Type II cutaneous mechanoreceptor (Ruffini corpuscle), thermoreceptor, muscle spindles are the receptors fires continuously as long as a stimulation is applied. Thus, option b, f, g, h are correct.
what are the role of receptors?
Receptors are the sensing protein molecule which bind to specific molecule in cell called as ligand.
The ligand can be a drug or any endogenous molecule like neurotransmitters, hormones, etc.
Based on molecular structure and mode of action four major types of receptors are found such as G-protein coupled receptors, ligand-gated ion channel receptors, Nuclear receptors, Kinase-linked receptors
Almost all the receptors have endogenous ligands such serotonin, acetylcholine etc.
Hence, option b, f, g, h are correct.
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Your question was incomplete. Hence the probable question was
Which receptor fires continuously as long as a stimulation is applied? Select all that apply.
a) Corpuscle of touch (Meissner corpuscle)
b) Type I cutaneous mechanoreceptor (Merkel disc)
c) Lamellated (Pacinian) corpuscle
d) Hair root receptor
e) Photoreceptor
f) Type II cutaneous mechanoreceptor (Ruffini corpuscle)
g) thermoreceptor
h) muscle spindles
The great alveolar cells secrete a phospholipid-protein mixture called pulmonary surfactant.
a. true
b. false
The __________ of pregnancy is called the anabolic phase because the mother is building up her stores of fat, nutrients, and glycogen.
The first half of pregnancy is called the anabolic phase because the mother is building up her stores of fat, nutrients, and glycogen.
What is anabolic phase?
Within 45 minutes after exercise, a crucial phase known as the anabolic phase begins. Muscle cells are very sensitive to insulin during this time, therefore it's important to consume the right nutrition if you want to increase your muscle endurance and strength.What is anabolic and catabolic process?
The creation of complex molecules from simpler ones is known as anabolism. These chemical processes call for energy. Complex molecules are broken down into smaller ones during catabolism. These processes produce energy.What is the primary growth stimulator of the fetus?
The main growth inducer for the fetus is insulin-like growth factor-1. A slowed fasting metabolism is a sign that the mother is in the catabolic phase of pregnancy.Learn more about anabolic phase
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Aside from having potent anti-inflammatory effects, glucocorticoids also promote homeostasis of which system?
Along with anti-inflammatory effects, glucocorticoids also promote homeostasis in T lymphocytes.
what are the role of glucocorticoid?
Glucocorticoids are steroid hormones have anti-inflammatory effects. as well as glucose homeostasis activity.
The hormones secreted from the cortex regulate glucose metabolism, synthesis in the adrenal cortex.
It promote gluconeogenesis in liver, but in skeletal muscle and white adipose tissue decrease glucose uptake, modulate the function of pancreatic α and β cells and help in to regulation of glucagon and insulin.
It also affect on glucose homeostasis to preserve plasma glucose for brain during stress.
Hence, glucocorticoids also promote homeostasis in T lymphocytes.
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Why are the exact timing and nature of anagenesis and cladogenesis difficult to infer from the fossil record?
We cannot infer exact timing and nature of anagenesis and cladogenesis from fossil record because,
There are gaps in the fossil record because it is difficult to achieve the conditions needed for fossilization.In comparison to species in other habitats, creatures that dwell where sediment is actively being deposited (such as beaches and wetlands) are more likely to become fossilized. Some organisms are more likely to decompose slowly and leave fossils behind, such as those with hard features like bones or shells.What is anagenesis? One taxon replaces another without branching in a single lineage as a result of evolutionary change is known as anagenesis.What is cladogenesis?The process by which a higher taxon or new group of animals emerges through evolutionary divergence like branching from an ancestor is known as cladogenesis.What is fossil record?Paleontologists, archaeologists, and geologists use the fossil record to put significant occurrences and species in the right geologic epoch. It is based on the Law of Superposition, according to which the lower layers of undisturbed rock sequences are older than the upper levels.The preserved remnants or traces of extinct animals, plants, and other species are known as fossils.This is because they demonstrate that earlier life on Earth was distinct from the species present now, fossils are crucial pieces of evidence for evolution.
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A model that describes the population of a fishery in which harvesting takes place at a constant rate is given by:_________
A model that describes the population of a fishery in which harvesting takes place at a constant rate is given by (dP/dt) = kP-h . In this k and h is positive constants.
The population growth model is based on the assumption that grows at rate of proportional to size of population . p is the population and k is relative growth rate. dp/dt is signal derived from pressure , typically ventricular blood pressure, indicated the change in pressure over time.
This model for population growth known as the logistic differential equation is dp/dt=kp. According to the population growth model that once population size exceeds available resources and population growth decrease dramatically.
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A prolonged period of drinking alcohol may result in damage to the ______ whereby formerly healthy cells are replaced with scar tissue.
Answer:
liver
Explanation:
prolonged abuse of alcohol disrupts the regeneration of liver cells leading to scar tissue (cirrhosis).
What is the role of glucose in respiration?
Answer:
One molecule of glucose goes through the process of cellular respiration to produce 36 ATP. Glucose is broken down to form pyruvate during the first step of cellular respiration, which is then used to fuel the citric acid cycle and oxidative phosphorylation.
Glucose is used to make energy in cellular respiration.
It produces the molecule ATP, the main energy storage and transfer unit of a cell.
Also, Cells this in order to remain healthy and active.
Explanation:
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Which hormone released by the highlighted structure inhibits the secretion of fsh only inhibin?
Inhibin hormone released by the highlighted structure inhibits the secretion of FSH only.
What is inhibin hormone?A protein called inhibin is secreted by the granulosa cells in women and the Sertoli cells in men. It decreases the amount of LH-releasing hormone in the hypothalamus and prevents the pituitary gland from producing and releasing follicle-stimulating hormone.Inhibin is a factor in the feedback control of FSH secretion in both males and females going through puberty. Follistatin suppresses FSH-subunit expression like inhibin, whereas activin enhances it, which in turn influences FSH production and secretion.Inhibin A is secreted by the corpora lutea and dominant ovarian follicles in women, which helps to explain why levels are so high during the late follicular and luteal stages. During the late luteal and early follicular phases of the menstrual cycle, inhibin B is reciprocally raised.Learn more about inhibin here:
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Question: Which hormone released by the highlighted structure inhibits the secretion of FSH only?